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Chapters
- 00:05Title
- 01:54Purification and Activation of B Cells
- 03:32Trypsinization, Fixation and Permeabilization
- 06:23Flourescence Staining for IgG1 and IgE Class Switching
- 08:37Results: Improvements Seen with Trypsinization
- 09:11Conclusion
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.
