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Chapters
- 00:05Title
- 01:43Brain Slice Preparation
- 02:49Patch-clamp Recordings from Layer 5 Pyramidal Neurons
- 05:35Injection of Conductance Through Simulated Synapses and Simulation of In Vivo-like Background Activity
- 06:47Results: Recreation of In Vivo-like Activity Using Dynamic Clamp
- 07:34Conclusion
Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.
