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Chapters
- 00:05Title
- 01:22Preparation of Stromal Cell-derived Factor-1α (SDF-1α) Glycan Nanoparticles
- 03:34Measurement of Particle Size and Zeta Potential
- 05:07Quantification of SDF-1α in the Particles
- 06:26In Vitro Release Assay
- 07:38Migration Assay
- 09:19Results: Analysis of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
- 11:23Conclusion
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
