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Chapters
- 00:05Title
- 01:36Synthesis of Tz-Bn-NOTA
- 03:43Preparing the huA33-TCO Immunoconjugate
- 05:2764Cu Radiolabeling of Tz-Bn-NOTA
- 07:04In Vivo Pretargeted PET Imaging
- 08:45Results: In Vivo Imaging of a Human Tumor Xenograft
- 10:12Conclusion
The bioorthogonal inverse electron demand Diels-Alder cycloaddition has been harnessed to create an effective and modular pretargeted PET imaging strategy for cancer. In this protocol, the steps of this methodology are described in the context of a model system employing the colorectal cancer targeted antibody huA33 and a 64Cu-labeled radioligand.
