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Chapters
- 00:05Title
- 01:24Determination of Growth-inhibitory Dose
- 03:48Systemic Chemical Genetic Screen
- 06:31Imaging Plates and Data Analysis
- 07:11Validation of Screen
- 09:25Results: Determination of Sub-inhibitory Concentration, a Chemical Genetic Screen, a 5-Fluorouracil, and Validation of Chemical Genetic Interactions
- 11:34Conclusion
Here we present a cost-effective method for defining chemical-genetic interactions in budding yeast. The approach is built on fundamental techniques in yeast molecular biology and is well suited for the mechanistic interrogation of small to medium collections of chemicals and other media environments.
