Journal
/
생물학
/
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

DOI:
10.3791/52357-v

•

08:46 min

•

January 06, 2015

•

, , , , , , , ,
1Oxford Protein Production Facility,Research Complex at Harwell, 2Protein Crystallography Group,Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory,Diamond Light Source

Chapters

  • 00:05Title
  • 01:58Analysis of Isopropyl Beta-D-1-thiogalactopyranoside (IPTG)-induced Expression by In-gel Flourescence
  • 03:37Scale-up of Cell Cultures and Membrane Preparation
  • 05:30Solubilization and Analysis of the Membrane Fraction
  • 06:48Results: Representative In-gel and FSEC Analyses
  • 08:11Conclusion

Summary

자동 번역

자동 번역

A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

Tags

Keywords: Membrane ProteinsRecombinant ExpressionGFP FusionE. ColiSDS-PAGEFSECSED ProteinsCell Division

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/52357?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/kr/52357?article_type=v

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
check_url/kr/52357?article_type=v

Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/kr/52357?article_type=v

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
check_url/kr/52357?article_type=v

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/kr/52357?article_type=v

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
check_url/kr/52357?article_type=v

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
check_url/kr/52357?article_type=v

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
check_url/kr/52357?article_type=v

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
check_url/kr/52357?article_type=v

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Read Article