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Chapters
- 00:05Title
- 01:15Preparation of 3D Cell Spheroids and Polyplex Nanomicelles
- 03:33Gene Transfection and Transplantation of Spheroids and Evaluation of Transgene Expression
- 05:38Results: Genetically-modified Cell Transplantation Using Gene Transfected Spheroids
- 07:14Conclusion
This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.
