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In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
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In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
DOI:

10:25 min

February 04, 2015

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Chapters

  • 00:05Title
  • 01:16Preparations
  • 03:22Extracting the Embryos
  • 05:49Injecting the Embryos
  • 08:29Results: Vascular Formation Precedes Flow in the Developing Kidney
  • 10:01Conclusion

Summary

자동 번역

This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.

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