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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

DOI:
10.3791/52404-v

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09:49 min

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April 02, 2015

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,
1School of Biological Sciences,Nanyang Technological University

Chapters

  • 00:05Title
  • 01:48Density Matching of Detergent Miscelles with 2H2O
  • 05:37Sedimentation Equilibrium of SH Protein in C14 Sulfobetaine Micelles
  • 08:30Results: Effect of pH on SH Protein Pentameric Stability
  • 09:08Conclusion

Summary

자동 번역

자동 번역

Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

Tags

Sedimentation EquilibriumAnalytical UltracentrifugationMembrane ProteinOligomerPentamerHistidine ProtonationProtein-protein InteractionAssociation ConstantStoichiometryThermodynamicsRespiratory Syncytial VirusSH Protein

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