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Chapters
- 00:05Title
- 01:48Density Matching of Detergent Miscelles with 2H2O
- 05:37Sedimentation Equilibrium of SH Protein in C14 Sulfobetaine Micelles
- 08:30Results: Effect of pH on SH Protein Pentameric Stability
- 09:08Conclusion
Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).
