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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

DOI:
10.3791/52419-v

•

05:20 min

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November 28, 2014

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, ,
1Cancer Immunology Program,Peter MacCallum Cancer Centre

Chapters

  • 00:05Title
  • 01:00Sample Preparation
  • 02:07Granzyme B Activity Assay
  • 03:30Results: The Ala-Ala-Asp-S-Benzyl Substrate is Specific for Granzyme B
  • 04:53Conclusion

Summary

자동 번역

자동 번역

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

Tags

Keywords: Granzyme BColorimetric AssayProteolytic ActivityBoc-Ala-Ala-Asp-S-BzlCytotoxic T LymphocytesNatural Killer CellsSerine ProteaseApoptosisSubstrate SpecificityRecombinant Proteases

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