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الكمي للمناعة tacrolimus على وبقع الدم الجافة عن طريق LC-MS / MS
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JoVE 신문 의학
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
DOI:

08:38 min

November 08, 2015

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Chapters

  • 00:05Title
  • 01:05Preparation of References, Solutions, Calibration Curve and Quality Control Samples
  • 03:52Collection of Clinical Samples and Extraction of Tacrolimus Dried Blood Spot Samples
  • 05:02LC-MS/MS Analysis and Quantification
  • 06:20Results: Analysis and Quantification of Tacrolimus on Dried Blood Spots by LC-MS/MS
  • 08:01Conclusion

Summary

자동 번역

Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.

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