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Direkte visualisering af den murine Dorsal Cochlear Nucleus for optogenetic Stimulering af Auditory Pathway
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Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Direkte visualisering af den murine Dorsal Cochlear Nucleus for optogenetic Stimulering af Auditory Pathway

DOI:
10.3791/52426-v

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07:58 min

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January 20, 2015

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, , , , , ,
1Department of Otology and Laryngology,Harvard Medical School, 2Department of Communication Sciences and Disorders,Worcester State University

Chapters

  • 00:05Title
  • 01:31Primary Craniotomy and Dorsal Cochlear Nucleus Exposure
  • 04:38Pressure Microinjection for Virus-mediated Gene Transfer and Surgical Recovery
  • 05:32Secondary Craniotomy and Cochlear Nucleus Exposure
  • 06:29Results: Secondary Cochlear Nucleus Exposure for Stimulation with Blue Light
  • 07:11Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to outline a surgical approach to provide direct access to the dorsal cochlear nucleus in a murine model.

Tags

Auditory PathwayDorsal Cochlear NucleusOptogenetic StimulationVirus-mediated Gene TransferPosterior Fossa ApproachOpsin InjectionNeural StimulationNeurophysiology Experiments

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