This content is Free Access.
1Institute of Pathology,University Medical Center, Göttingen, 2Department of Hematology/Oncology,Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group,Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry,University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center
Chapters
- 00:05Title
- 01:20Cell Enrichment
- 02:46Protein Extraction
- 03:59Protein Concentration Measurement and Spike-in
- 05:01Liquid Chromatography and Mass Spectrometric Analysis
- 06:10Results: Representative Mass Spectrometric Analysis of Liquid and Tumor Cell Samples
- 07:08Conclusion
In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.
