JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:01Intravitreal Injection of AAV
- 03:52Immunohistochemistry: Retinal Cryosections
- 05:04Immunohistochemistry: Retinal Flatmounts
- 06:15Immunohistochemistry: Labeling, Mounting, and Imaging
- 07:24PCR Analysis of Mouse Blood Samples
- 08:24Results: Retinal Permeability in Dp71-null Mice
- 09:40Conclusion
To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.
