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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

DOI:
10.3791/52482-v

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12:28 min

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May 03, 2015

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, ,
1Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Chapters

  • 00:05Title
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

자동 번역

자동 번역

This manuscript describes the detection of sumoylation and ubiquitination of kinetochore proteins, Ndc10 and Ndc80, in the budding yeast Saccharomyces cerevisiae.

Tags

Protein PurificationSumoylationUbiquitinationKinetochore ProteinsNdc10Ndc80Post-translational ModificationsSaccharomyces Cerevisiae

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