This content is Free Access.
Chapters
- 00:05Title
- 01:14Crypt Isolation from Whole Tissue
- 03:31Crypt Culture in Basement Membrane Matrix
- 04:27Results: Human Epithelial and Colonoids Cultured from Whole Tissue and Biopsy
- 05:57Conclusion
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
