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Generatie van de Mens Adipose stamcellen via dedifferentieëren Mature Adipocyten in Plafond Culturen
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
DOI:

07:35 min

March 07, 2015

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Chapters

  • 00:00Title
  • 00:17Introduction
  • 00:54Methods
  • 06:00Results
  • 06:56Conclusion

Summary

Automatic Translation

Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

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