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A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
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JoVE 신문 의학
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
DOI:

09:30 min

February 21, 2015

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Chapters

  • 00:05Title
  • 01:22Harvest of Neural Rosettes
  • 03:03Expansion of Neural Progenitor Cells
  • 04:27NPC Transduction
  • 05:15Neurosphere Migration Assay
  • 07:06Results: Patient-specific hiPSCs can be Differentiated into Healthy NPCs and Neurons
  • 08:56Conclusion

Summary

자동 번역

This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.

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