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Manipulatie en<em> In Vitro</em> Rijping van<em> Xenopus laevis</em> Eicellen, Gevolgd door intracytoplasmatische sperma-injectie, om embryonale ontwikkeling
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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
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Manipulatie en<em> In Vitro</em> Rijping van<em> Xenopus laevis</em> Eicellen, Gevolgd door intracytoplasmatische sperma-injectie, om embryonale ontwikkeling

DOI:
10.3791/52496-v

•

09:22 min

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February 09, 2015

•

, ,
1Wellcome Trust/Cancer Research UK Gurdon Institute,University of Cambridge, 2Department of Zoology,University of Cambridge

Chapters

  • 00:05Title
  • 01:14Preparations
  • 03:47Injecting Oocytes with Oligos and Oocyte Maturation
  • 05:55Intracytoplasmic Sperm Injection
  • 08:12Results: ICS Embryo Survival
  • 09:01Conclusion

Summary

자동 번역

자동 번역

We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

Tags

Xenopus LaevisOocytesIn Vitro MaturationIntracytoplasmic Sperm InjectionICSIEmbryonic DevelopmentMaternal FactorsGene InterferenceAntisense OligonucleotidesMRNA InjectionProtein ExpressionSwimming Tadpole

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