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使用小鸡GFP 神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
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Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
DOI:

09:57 min

May 28, 2015

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Chapters

  • 00:05Title
  • 01:28Preparing the Host Embryo
  • 03:30Preparing the Donor Graft Tissue
  • 05:05Grafting the Tissue
  • 06:11Injecting Dil into Blood Vessels
  • 07:20Harvest Embryos for Sectioning or Wholemount Examination
  • 08:05Results: Dual Labeling in Chimeric Chick Embryos
  • 09:19Conclusion

Summary

자동 번역

在这里,我们报告神经波峰细胞和血管的双重标签使用小鸡GFP 神经管内部嫁接结合血管内DII注射。这种实验技术使我们能够同时在器官发生期间可视化和研究NCC衍生(肠)神经系统和血管系统的发展。

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