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染色质监管机构和各国的全基因组快照<em>爪</em>通过胚胎的ChIP-SEQ

JoVE 신문
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Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

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染色质监管机构和各国的全基因组快照<em>爪</em>通过胚胎的ChIP-SEQ

DOI:

10.3791/52535-v

•

10:23 min

•

February 26, 2015

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George E. Gentsch, Ilya Patrushev, James C. Smith

¹Division of Systems Biology,MRC National Institute for Medical Research

Chapters

00:05Title
01:37Chromatin Cross-linking
02:55Chromatin Extraction
04:31Chromatin Fragmentation
06:07Chromatin Immunoprecipitation
08:16Results: Chromatin Binding of the T-box Transcription Factor VegT at Gastrula Stage of X. tropicalis Embryos
09:54Conclusion

Summary

자동 번역

如何染色质监管机构和染色质状态的问题会影响体内的基因组是关键，我们的细胞命运决定是如何在早期发育中的胚胎制成的认识。芯片起，最流行的做法在全球范围内，在这里介绍的爪蟾胚胎研究染色质的功能。

Tags

Chromatin Regulators Chromatin States Xenopus Embryos ChIP-Seq Chromatin Immunoprecipitation Genome-wide Profiling DNA-binding Proteins Transcription Factors Chromatin Modifiers Histone Modifications Embryogenesis Model Organisms Genome Assembly

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