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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
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JoVE 신문 신경과학
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
DOI:

08:37 min

May 28, 2015

Chapters

  • 00:05Title
  • 01:09Neural Dissociation
  • 03:14Myelin Depletion
  • 04:50FACS Staining
  • 06:27Results: Sorting Neurons, Astrocytes, and Microglia from Individual Samples
  • 08:11Conclusion

Summary

자동 번역

The goal of this protocol is to use the fluorescence activated cell sorting (FACS) technique to sort specific types of neural cells for subsequent analysis of cell-type-specific gene expression, epigenetic markers, and or protein expression.

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