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斑马鱼肾功能评价采用荧光测定间隙

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Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

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斑马鱼肾功能评价采用荧光测定间隙

DOI:

10.3791/52540-v

•

08:13 min

•

February 20, 2015

•

Sonia Christou-Savina, Philip L. Beales, Daniel P. S. Osborn

¹Genetics and Genomic Medicine, Institute of Child Health,University College London, ²Molecular Cell Science Research Centre,St. George’s University of London

Chapters

00:05Title
01:09Preparation of Instruments and Anesthetic
02:21Zebrafish Husbandry and Pre-injection Treatment
03:33Microinjection
05:27Imaging Acquisition and Processing
07:08Results: Injection of Rhodamine Dextran in bbs9 Morpholino Knockdown Zebrafish
07:37Conclusion

Summary

자동 번역

斑马鱼是一种流行的工具来模拟慢性肾脏病（CKD）。然而，它们的小尺寸使得它不可能用传统方法评估肾功能。我们描述一个荧光染料肾清除率法^1，允许在CKD斑马鱼肾功能定量分析。

Tags

Keywords: Zebrafish Kidney Fluorescent Clearance Assay Nephrons Pronephric Tubules Cilia Kidney Function Chronic Kidney Disease Optical Transparency Renal Function

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