Journal
/
발생학
/
Automatiseret Kvantificering af hæmatopoietisk celle - stromacelle Interaktioner i Histologiske Billeder af afkalkede Bone
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automatiseret Kvantificering af hæmatopoietisk celle - stromacelle Interaktioner i Histologiske Billeder af afkalkede Bone

DOI:
10.3791/52544-v

•

09:31 min

•

April 08, 2015

•

, , , ,
1Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine

Chapters

  • 00:05Title
  • 01:14Femoral Bone Cryosectioning
  • 02:28Imaging
  • 04:02Random Bone Marrow Positioning Simulation
  • 07:36Results: Representative Analysis of the Bone Marrow Niche Microenvironments for Long Lived Plasma Cells
  • 09:00Conclusion

Summary

자동 번역

자동 번역

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

Tags

Automated QuantificationCell-cell InteractionsHematopoietic CellsStromal CellsBone MarrowConfocal MicroscopyHistological AnalysisCell RecognitionNeighborhood AnalysisCo-localizationRandom Positioning

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/52544?article_type=v

Kvantificering af Colon stamcelle Mutationer
check_url/kr/52544?article_type=v

Konkurrencedygtige Transplantationer at vurdere hæmatopoietisk stamcelletransplantation Fitness
check_url/kr/52544?article_type=v

Temporal Bestilling af Dynamic Expression data fra Detaljeret Spatial Expression Maps
check_url/kr/52544?article_type=v

Isolering og karakterisering af Mesenchymale stromacellers fra human navlestrengen og føtalt Placenta
check_url/kr/52544?article_type=v

En ny mammedisk fedtpudstransplantationsteknik til visualisering af fartøjsgenerering af vaskulære endotelceller
check_url/kr/52544?article_type=v

Bioengineering af humaniserede knoglemarv mikro miljøer i mus og deres visualisering ved Live Imaging
check_url/kr/52544?article_type=v

Hornhindevæv Engineering: En In Vitro Model af den menneskelige hornhinden stromale-nerve interaktioner
check_url/kr/52544?article_type=v

En RANKL-baserede osteoklast kultur Assay af musen knoglemarven til at undersøge rollen, som mTORC1 i osteoklast dannelse
check_url/kr/52544?article_type=v

Encellede kvantificering af Protein nedbrydning satser for Time-Lapse Fluorescens mikroskopi i vedhængende cellekultur
check_url/kr/52544?article_type=v

Klonede analyse af embryonale hæmatopoietisk stamcelle prækursorer ved hjælp af enkelt celle indeks sortering kombineret med endotel celle Niche Co kultur

Read Article