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Chapters
- 00:05Title
- 01:14Femoral Bone Cryosectioning
- 02:28Imaging
- 04:02Random Bone Marrow Positioning Simulation
- 07:36Results: Representative Analysis of the Bone Marrow Niche Microenvironments for Long Lived Plasma Cells
- 09:00Conclusion
A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.
