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Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

DOI:
10.3791/52551-v

•

09:24 min

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April 22, 2015

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, , ,
1Hans Berger Department of Neurology,Jena University Hospital

Chapters

  • 00:05Title
  • 02:17Injection of Thymidine Analogs and Tissue Preparation
  • 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
  • 06:27Imaging and Data Analysis
  • 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
  • 08:42Conclusion

Summary

자동 번역

자동 번역

This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

Tags

Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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