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Chapters
- 00:05Title
- 01:38Conditioning of Recipient Mice and Isolation of Donor Bone Marrow Cells
- 06:17Bone Marrow Transplantation and Estimating the Extent of Bone Marrow Chimerism
- 09:06Results: Quantification of Bone Marrow Chimerism in Busulfan-conditioned Mice
- 10:49Conclusion
We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.
