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Forward Génétique écrans à l'aide pour identifier les macrophages<em> Toxoplasma gondii</em> gènes importants pour la résistance à l'IFN-γ-cellulaire dépendant immunité autonome
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JoVE 신문 면역학 및 감염병학
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
DOI:

11:21 min

March 12, 2015

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Chapters

  • 00:05Title
  • 02:15Isolation of Parasite Mutants with a Fitness Defect Following Activation of Infected Macrophages
  • 06:03Evaluation of Mutants to Determine if the Defect is at the Level of Parasite Survival or Replication Following Activation of Infected Macrophages
  • 09:16Results: T. gondii Mutants with Fitness Defects are Identified by Phase Contrast and Immunofluorescence Microscopy
  • 10:48Conclusion

Summary

자동 번역

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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