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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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JoVE 신문 생체공학
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

DOI:
10.3791/52576-v

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08:35 min

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February 26, 2015

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, , , ,
1Department for Tissue Engineering and Regenerative Medicine,University Hospital Würzburg, 2Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease,Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB

Chapters

  • 00:05Title
  • 01:55Production of Dermal Component
  • 03:36Addition of Human Epidermal Keratinocytes and Culture of Full Thickness Skin Equivalents
  • 05:14Injury with Automated Wounding Device
  • 06:19Results: (Immuno-)Histological Analysis and Wounding of Full Thickness Skin Equivalents
  • 08:10Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.

Tags

3D Skin EquivalentFull Thickness Skin ModelIn Vitro Wound HealingHuman Dermal FibroblastsHuman Epidermal KeratinocytesCollagen Type I HydrogelAutomated Wounding DeviceStandardized Wounds

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