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自噬的分析<em>产黄青霉</em>通过结合使用饥饿垫荧光显微镜
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Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy
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自噬的分析<em>产黄青霉</em>通过结合使用饥饿垫荧光显微镜

DOI:
10.3791/52577-v

•

06:51 min

•

February 01, 2015

•

1LOEWE Excellence Cluster for Integrative Fungal Research (IPF),Senckenberg Research Institute

Chapters

  • 00:05Title
  • 01:06P. chrysogenum Preparation
  • 02:16Starvation Pad Cultivation Preparation
  • 03:22P. chrysogenum Starvation Pad Inoculation and Incubation
  • 04:25Microscopic Sample Analysis
  • 05:11Results: Representative GFP-SKL Localization
  • 06:28Conclusion

Summary

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자동 번역

A convenient and powerful method for studying autophagy in Penicillium chrysogenum by using starvation pads (mixtures of agarose and tap water in a microscope slide containing a central cavity) is presented here.

Tags

Keywords: AutophagyPenicillium ChrysogenumStarvation PadsFluorescence MicroscopyCellular Quality ControlOrganelle DynamicsProtease ActivityFilamentous FungusMicroscopy Evaluation

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