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1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen
Chapters
- 00:05Title
- 01:59Eye Enucleation and Isolation of Retina
- 03:22Slice Preparation
- 04:59Two-photon Microscopy
- 06:14Light Stimulation and Recording
- 06:53Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
- 08:10Conclusion
Descriviamo un protocollo per il monitoraggio Ca 2+ dinamiche nei terminali degli assoni dei fotorecettori cono con un ex-vivo la preparazione fetta della retina del mouse. Questo protocollo permette studi completi di cono Ca 2 + Segnalazione di un importante sistema modello mammiferi, il mouse.
