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Chapters
- 00:05Title
- 01:28Flow Cell Setup and Inoculation
- 03:54Characterizing Biofilm Development in Response to Nutrient Gradients Using Confocal Miscroscopy
- 05:12Characterizing Internal Solute Transport by Injections of Conservative Fluorescent Tracer
- 06:39Characterizing the Surrounding Flow Field by Tracking Fluorescent Particles
- 07:37Representative Analysis of Bacterial Interactions in Biofilms Under Glucose Gradients
- 08:33Conclusion
Biofilms have complex interactions with their surrounding environment. To comprehensively investigate biofilm-environment interactions, we present here a series of methods to create heterogeneous chemical environment for biofilm development, to quantify local flow velocity, and to analyze mass transport in and around biofilm colonies.
