JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:10Mouse Breast Tumor Primary Cell Preparation
- 02:33Lung Metastasis Visualization by Mouse Lung Extraction
- 03:25Mouse Embryonic Fibroblast (MEF) Preparation
- 04:52Results: Representative Primary Tumor Cell and MEF Isolation
- 05:51Conclusion
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
