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العدوى البكتيرية النظامية والمناعة الظواهر الدفاع في<em> الدروسوفيلاميلانوجاستر</em
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Systemic Bacterial Infection and Immune Defense Phenotypes in Drosophila Melanogaster
DOI:

10:12 min

May 13, 2015

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Chapters

  • 00:05Title
  • 02:01Bacteria Preparation
  • 02:55Fly Infection by Nanoinjector
  • 04:49Fly Injection by Septic Pinprick
  • 05:49Assaying Bacterial Load Post-infection
  • 08:02Results: Bacterial Infection of Drosophila melanogaster
  • 09:15Conclusion

Summary

자동 번역

Drosophila melanogaster is an outstanding model organism for studying innate immune systems and the physiological consequences of infection and disease. This protocol describes how to deliver robust and quantitatively repeatable bacterial infections to D. melanogaster, and how to subsequently measure infection severity and quantify the host immune response.

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