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3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
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3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

DOI:
10.3791/52614-v

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06:47 min

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July 30, 2015

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, , , ,
1Division of Developmental Immunology,German Cancer Research Center (DKFZ), 2Genetics of Skin Carcinogenesis,German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 01:163D Organotypic Co-cultures (OTCs)
  • 02:46mTEC Co-culture
  • 05:14Results: Growth Patterns and Proliferation of mTECs within the OTCs
  • 06:10Conclusion

Summary

자동 번역

자동 번역

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

Tags

Keywords: 3D Organotypic Co-culture ModelMedullary Thymic Epithelial Cells (mTECs)Promiscuous Gene ExpressionT Cell DevelopmentSelf-toleranceRANKLFoxN1AireCortical Thymic Epithelial Cells (cTECs)

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