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Chapters
- 00:05Title
- 01:30Assembling the Quantitative Recombinase Polymerase Amplification (qRPA) Reactions for a Standards Curve
- 05:16Constructing a Standard Curve from Multiple Experiments with Custom Scripts
- 06:55Results: Construction of an HIV-1 DNA Standard Curve by qRPA
- 07:53Conclusion
Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.
