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Chapters
- 00:05Title
- 02:25Synthesis of Manganese Prussian Blue (MnPB) Nanoparticles
- 04:09Biofunctionalization and Characterization of MnPB Nanoparticles
- 05:29Fluorescent Labeling of Cells with Nanoparticles
- 06:59Generating MRI Contrast on Cells with Nanoparticles
- 09:01Results: Imaging of Cells Labeled with Prussian Blue Nanoparticles
- 10:34Conclusion
This protocol describes the synthesis of biofunctionalized Prussian blue nanoparticles and their use as multimodal, molecular imaging agents. The nanoparticles have a core-shell design where gadolinium or manganese ions within the nanoparticle core generate MRI contrast. The biofunctional shell contains fluorophores for fluorescence imaging and targeting ligands for molecular targeting.
