JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:56Passaging of hiPSCs
- 04:52Cardiac Differentiation of Human iPSC
- 07:17Purification of Human Cardiomyocytes through Glucose Starvation
- 09:39Representative Analysis of Differentiated Cardiomyocytes
- 10:55Conclusion
Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.
