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LEPR<sup> DB</sup> 2型糖尿病のマウスモデル:無傷の膵島の膵島単離およびライブセル2光子イメージング
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Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
DOI:

10:09 min

May 11, 2015

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Chapters

  • 00:05Title
  • 01:31Reagent Preparation
  • 03:22Islet Isolation and Islet Culturing
  • 06:50Live Cell Photon Imaging
  • 08:06Results: Pancreatic Islet Yield and Insulin Secretion Using 2-Photon Assay
  • 09:37Conclusion

Summary

자동 번역

We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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