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Chapters
- 00:05Title
- 01:21Isolation and Cleanup of Total RNA
- 02:58cDNA Preparation and Labeling
- 04:54cDNA Hybridization
- 06:59Slide Washing
- 08:02Results: Bacterial Transcriptome Analysis through Serine-dependent Gene Regulation
- 09:39Conclusion
This manuscript describes the use of state-of-the-art technology provided by DNA-microarrays. Microarrays provide an overview of the transcriptomic changes in bacteria incurred under a specific condition. Moreover, we highlight the ease by which large amounts of data can be analyzed by using convenient in-house developed software packages.
