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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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Chapters
- 00:05Title
- 01:28Stimulation and Collection of Microparticles
- 03:26Characterization of Microparticles via FACS Analysis
- 05:10Preparation of Bronchial Tissue Explants
- 06:44Lymphocytic Microparticle Treatment
- 07:51Results: Lymphocytic Microparticle Detection and Analysis
- 08:57Conclusion
Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.
