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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
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Chapters
- 00:05Title
- 01:07DNA Preparation
- 02:41Gene Gun Operation
- 04:59Plating Transformed Cells
- 05:52Results: Successful Homologous Recombination
- 07:04Conclusion
Biolistic transformation is a method used to generate stable integration of DNA into the genome of the opportunistic pathogen Cryptococcus neoformans through homologous recombination. We will demonstrate biolistic transformation of a construct, which has the gene encoding acetate kinase fused to the fluorescent tag mCherry into C. neoformans.
