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ุงู„ุชุญูˆู„ Biolistic ู…ู† ู†ูŠูˆู† ู…ุนู„ู… ุฌูŠู† ุฅู„ู‰ ุงู„ู‚ู†ุงุต ุงู„ูุทุฑูŠุฉ ู…ุณุจุจุงุช ุงู„ุฃู…ุฑุงุถ<em> ุงู„ู…ุณุชุฎููŠุฉ ุงู„ู…ูˆุฑู…ุฉ</em
JoVE ์‹ ๋ฌธ
๋ฉด์—ญํ•™ ๋ฐ ๊ฐ์—ผ๋ณ‘ํ•™
JoVE ๋น„๋””์˜ค๋ฅผ ํ™œ์šฉํ•˜์‹œ๋ ค๋ฉด ๋„์„œ๊ด€์„ ํ†ตํ•œ ๊ธฐ๊ด€ ๊ตฌ๋…์ด ํ•„์š”ํ•ฉ๋‹ˆ๋‹ค.  ์ „์ฒด ๋น„๋””์˜ค๋ฅผ ๋ณด์‹œ๋ ค๋ฉด ๋กœ๊ทธ์ธํ•˜๊ฑฐ๋‚˜ ๋ฌด๋ฃŒ ํŠธ๋ผ์ด์–ผ์„ ์‹œ์ž‘ํ•˜์„ธ์š”.
JoVE ์‹ ๋ฌธ ๋ฉด์—ญํ•™ ๋ฐ ๊ฐ์—ผ๋ณ‘ํ•™
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
DOI:

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07:32 min

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March 19, 2015

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Chapters

  • 00:05Title
  • 01:07DNA Preparation
  • 02:41Gene Gun Operation
  • 04:59Plating Transformed Cells
  • 05:52Results: Successful Homologous Recombination
  • 07:04Conclusion

Summary

์ž๋™ ๋ฒˆ์—ญ

ุงู„ุชุญูˆู„ Biolistic ู‡ูˆ ุทุฑูŠู‚ุฉ ุชุณุชุฎุฏู… ู„ุชูˆู„ูŠุฏ ุงู„ุชูƒุงู…ู„ ู…ุณุชู‚ุฑ ู…ู† ุงู„ุญู…ุถ ุงู„ู†ูˆูˆูŠ ููŠ ุงู„ุฌูŠู†ูˆู… ู…ู† ุงู„ุงู†ุชู‡ุงุฒูŠุฉ ุงู„ุฃุฏุนูˆู…ูŠุฉ ุงู„ู…ู…ุฑุถ ุงู„ู…ุณุชุฎููŠุฉ ู…ู† ุฎู„ุงู„ ุฅุนุงุฏุฉ ุงู„ุชุฑูƒูŠุจ ู…ุซู„ูŠ. ูˆุณูˆู ู†ุธู‡ุฑ ุงู„ุชุญูˆู„ biolistic ู…ู† ุจู†ุงุกุŒ ูˆุงู„ุชูŠ ู„ุฏูŠู‡ุง ุฎู„ุงุช ูƒูŠู†ุงุฒ ุชุฑู…ูŠุฒ ุงู„ุฌูŠู†ุงุช ุชู†ุตู‡ุฑ ุฅู„ู‰ ุงู„ุนู„ุงู…ุฉ ูู„ูˆุฑูŠ mCherry ุฅู„ู‰ C. ุงู„ุฃุฏุนูˆู…ูŠุฉ.

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