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Chapters
- 00:05Title
- 01:36Clonogenic Assay Plate Preparation
- 04:13Clonogenic Assay
- 06:34Results: Pi3 Kinase Inhibitors Activate the Doormant Phenotype in Estrogen Sensitive Cells
- 07:59Conclusion
We developed an in vitro model of dormancy in the bone marrow for estrogen-sensitive breast cancer cells. The goal of this protocol is to demonstrate use of the model for the study of the molecular and cellular biology of dormancy and for generation of hypotheses for subsequent testing in vivo.
