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Die Quantifizierung der neurovaskulären Schutz Nach Repetitive hypoxische Präkonditionierung und Transient mittleren Hirnarterie Occlusion in Mice
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Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
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Die Quantifizierung der neurovaskulären Schutz Nach Repetitive hypoxische Präkonditionierung und Transient mittleren Hirnarterie Occlusion in Mice

DOI:
10.3791/52675-v

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09:48 min

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May 04, 2015

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, , , , , ,
1Department of Neurology and Neurotherapeutics,University of Texas Southwestern Medical Center, 2Department of Neurological Surgery,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:37Repetitive Hypoxic Preconditioning
  • 03:01Evans Blue (EB) Injection
  • 04:222,3,5-Triphenyltetrazolium Chloride (TTC) Staining
  • 08:12Results: RHP Reduces Infarct Volumes of Blood-brain Barrier (BBB) Disruption
  • 08:59Conclusion

Summary

자동 번역

자동 번역

This protocol describes repetitive hypoxic preconditioning, or brief exposures to systemic hypoxia that reduce infarct volumes and blood-brain barrier disruption following transient middle cerebral artery occlusion in mice. It also details dual quantification of infarct volume and blood-brain barrier disruption after stroke to assess the efficacy of neurovascular protection.

Tags

Keywords: Neurovascular ProtectionRepetitive Hypoxic PreconditioningTransient Middle Cerebral Artery OcclusionInfarct VolumeBlood-brain Barrier DisruptionIschemic StrokeMouse ModelCentral Nervous SystemNeuroprotectionPro-inflammatory Disease

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