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Chapters
- 00:05Title
- 01:16Preparation of Urokinase and Enzymatic Digestion of Clot
- 03:13Seeding for Expansion of Cell Culture and CFU Plates
- 04:13Giemsa Stain for CFU Assay
- 05:00Canine MSC Differentiation
- 06:52Results: Mesenchymal Stem Cell Rescue from Clotted Bone Marrow
- 08:17Conclusion
Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.
