This content is Free Access.
1Department of Orthopaedic Surgery,University of Michigan Medical School, 2Department of Molecular & Integrative Physiology,University of Michigan Medical School, 3Department of Biomedical Engineering,University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School
Chapters
- 00:05Title
- 01:29Pre-sample Preparation
- 02:25Skeletal Muscle Bundle Dissection, Permeabilization, and Storage
- 04:48Preparation for Single Fiber Testing
- 07:41Single Fiber Testing
- 09:51Results: Activation Rate of Slow and Fast Fibers During Stimulation
- 10:56Conclusion
Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. In this article we outline a valid and reliable technique to prepare and test permeabilized skeletal muscle fibers in vitro.
