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1Department of Orthopaedic Surgery,University of Michigan Medical School, 2Department of Molecular & Integrative Physiology,University of Michigan Medical School, 3Department of Biomedical Engineering,University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School
Chapters
- 00:05Title
- 01:29Pre-sample Preparation
- 02:25Skeletal Muscle Bundle Dissection, Permeabilization, and Storage
- 04:48Preparation for Single Fiber Testing
- 07:41Single Fiber Testing
- 09:51Results: Activation Rate of Slow and Fast Fibers During Stimulation
- 10:56Conclusion
Análisis de las propiedades contráctiles de las fibras musculares, esqueléticas químicamente piel o permeabilizadas ofrece un poderoso medio de la cual evaluar la función muscular a nivel de la célula muscular individual. En este artículo describimos una técnica válida y fiable para preparar y probar permeabilized fibras musculares esqueléticas in vitro.
