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1Department of Orthopaedic Surgery,University of Michigan Medical School, 2Department of Molecular & Integrative Physiology,University of Michigan Medical School, 3Department of Biomedical Engineering,University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School
Chapters
- 00:05Title
- 01:29Pre-sample Preparation
- 02:25Skeletal Muscle Bundle Dissection, Permeabilization, and Storage
- 04:48Preparation for Single Fiber Testing
- 07:41Single Fiber Testing
- 09:51Results: Activation Rate of Slow and Fast Fibers During Stimulation
- 10:56Conclusion
Kimyasal tenli, ya da permeabilize, iskelet kas liflerinin kasılma özelliklerinin analizi tek kas hücre düzeyinde kas fonksiyonunu değerlendirmek için bir güçlü bir araç sunuyor. Bu yazıda hazırlamak için geçerli ve güvenilir bir teknik anahat ve test in vitro iskelet kas lifleri geçirgenleştirildi.
